
Atherosclerosis
Our proprietary APOE*3-Leiden and APOE*3-Leiden.CETP models closely reflect human lipoprotein metabolism and development of atherosclerotic cardiovascular disease. These mice develop diet-induced hyperlipidaemia, vascular inflammation, and progressive atherosclerotic lesions that respond to treatment in a clinically predictive manner. The model has been extensively validated with a wide range of approved and emerging interventions, including statins, fibrates, ezetimibe, GLP-1 receptor agonists, and PCSK9, ANGPTL3, and CETP inhibitors. In addition to atherosclerosis progression and plaque regression studies, we offer dedicated exploratory lipid and lipoprotein profiling studies that provide rapid proof-of-concept data on compound efficacy. This makes the APOE*3-Leiden(.CETP) models a powerful translational tool for evaluating novel therapies targeting dyslipidaemia and cardiovascular disease.
Key capabilities
Study types
Tailor-made efficacy studies for targeted pathways and mechanisms
Models
APOE*3-Leiden and APOE*3-Leiden.CETP
Output
Quantitative lesion data & translational endpoints

A powerful approach to accelerate cardiovascular drug development
TNO supports pharmaceutical and biotech organisations in evaluating the efficacy of novel compounds targeting cardiovascular disease. By combining translational in vivo models with quantitative histopathology, we provide deep insights into lesion development, progression, and treatment response. Our approach allows you to assess compound performance under physiologically relevant conditions, benchmark against existing therapies, and generate robust datasets that support early decision-making in the development pipeline.
30+
years of cardiovascular preclinical expertise
200+
completed efficacy studies across disease indications
100+
pharma & biotech partners
250+
peer-reviewed publications
A powerful model to accelerate cardiovascular drug development
TNO supports pharmaceutical and biotech organisations in evaluating the efficacy of novel compounds targeting cardiovascular disease. By combining translational in vivo models with quantitative histopathology, biochemistry and molecular profiling, we provide deep insights into lesion development, progression, and treatment response. Our approach allows you to assess compound performance under physiologically relevant conditions, benchmark against existing therapies, and generate robust datasets that support early decision-making in the development pipeline.
Use cases

Lipid and lipoprotein profiling studies
Rapid proof-of-concept assessment of compound efficacy on lipids and lipoproteins.

Drug efficacy testing
Evaluate the effects of interventions on atherosclerosis progression or regression.

Mechanism of action studies
Investigate how compounds influence metabolic, inflammatory, and organ crosstalk pathways.
Choose the right preclinical research partner
TNO bridges the gap between preclinical research and clinical outcomes. Our models are continuously validated against clinical data, ensuring maximal translational relevance.
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Clinically predictive translational models
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Holistic understanding of cardiovascular disease mechanisms
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Ethical and responsible research execution
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Clear, actionable data to support decision-making

Ready to accelerate your atherosclerosis research?
Get in touch to discuss your research question and explore how TNO can design and execute a tailored preclinical obesity for your compound. In an initial discussion, we:
- align on your research objective
- propose suitable model-diet combinations and study designs
- provide a first outline and indicative cost range

